Environmental Quality Laboratory Instrumentation

Organic Analysis

An EQL employee works on laboratory equipment

Agilent 1200 high-performance liquid chromatography (HPLC)

Analysis of non-volatile organic compounds in water (reverse phase chromatography)

Diode array and fluorescence detectors

Pharmaceutical and personal care products analysis

Limit of detection in μg/L range

An EQL employees works on the Abraxis CAAS automated contaminant analysis system

Abraxis CAAS automated contaminant analysis system

Fully automated 96-well microplate analysis system: fluid handling, plate mixing, incubation timing, optical reading, calculating, and reporting

Uses enzyme-linked immunosorbent assay (ELISA) kits for analysis of :

  • Nodularin + microcystins
  • Anatoxin-a
  • Cylindrospermopsin
  • Saxatoxins
  • Assay kits also available for
  • Pesticides, herbicides
  • PCBs
An EQL employee works on the Agilent 6495D triple quadrupole mass spectrometer

Agilent 6495D triple quadrupole mass spectrometer / 1290 Infinity ultrahigh performance liquid chromatograph

Analysis of 40 + PFAS compounds in various matrices in a PFAS free system minimizing background noise and contamination

PFAS MRM date base for rapid method development and quantitation

High sensitivity

Application focused tuning and workflow facilitate analysis and quantitation of personal care products and pharmaceuticals, lipids, metabolites, and pesticides in addition of PFAS

Detection levels at ng/L levels

A EQL employee works with laboratory equipment

Agilent 7890A Gas chromatograph 5975C electron impact (EI)/chemical ionization (CI) mass spectrometer

Direct liquid injection or headspace analysis with a CTC PAL autosampler system for the analysis of volatile and semi-volatile organic compounds

Frontier Laboratories Multi-shot micro furnace pyrolyzer

Characterization of natural organic matter and humic materials

Identify and quantify different types of plastic polymers

High sensitivity and low background results in 0.1 ug detection limits

An EQL employee works on the Shimadzu TOC-L, TNM

Shimadzu TOC-L, TNM-L total organic carbon and total nitrogen analyzer

Analysis of total organic carbon or dissolved organic carbon in water samples with a working range from 5 μg/L to 10,000 mg/L carbon

Analysis of total nitrogen in water samples usingchemiluminescence detection

Inorganic Analysis

ThermoScientific Dioxex Integrion

ThermoScientific Dionex Integrion high pressure ion chromatograph

Analysis of major cations and anions in water

Analysis of low molecular weight organic acids in water

Conductivity detector

Automated eluent generation

Agilent 8900 Triple quadrupole ICP-MS

Agilent 8900 Triple quadrupole ICP-MS

Analysis of 30+ elements in water, soil, sediment, tissue samples

High sensitivity and low background results in ng/L detection limits

MS/MS ensures reliable results for difficult elements such as Si, P, and S

MS/MS analysis of raw earth elements

Stable isotope analysis

Particle size analysis of microplastics by single particle (sp) ICPMS

Characterization of metal nanoparticles by sp-ICPMS

Spectroscopy

Shimadzu UV-2600i

Shimadzu UV-2600i UV-Vis spectrophotometer with integrating sphere

Scanning range 185-900 nm

Integrating sphere allows generation of spectrum for films, gels, creams, and metallic foils

SpectraMax iD3 Plate Reader

SpectraMax iD3 Plate Reader

High-throughput absorbance, fluorescence, and luminescence assays

Horiba Aqualog

Horiba Aqualog

Performs A-TEEM spectroscopy, simultaneous Absorbance, Transmittance, and Fluorescence Excitation-Emission-Matrix (EEMs).

  • Measuring colored dissolved organic matter (CDOM) composition
  • oil-in-water analysis
  • benzene, toluene, ethylbenzene and xylene (BTEX) analysis
  • identifying and quantifying complex biomolecules

Microbiology

Bio-Rad CFX Opus 96

Bio-Rad CFX Opus 96 qPCR System

Real-time gene quantification and microbial community profiling with qPCR (e.g., ARGs, HABs)

ECHO Revolve 4

ECHO Revolve 4, Hybrid Fluorescence Microscope

Fluorescence imaging of microbial and eukaryotic cells; biofilm and cell physiology studies

NanoBrook 90 Plus PALS

NanoBrook 90Plus PALS

NanoBrook 90Plus PALS (phase analysis light scattering)

Dynamic light scattering for particles/molecules size over the range of 0.3 nm-6 μm at 90° and 15°

Phase light scattering for particle/molecular surface charge determination (zeta potential)

Sample Preparation & Additional Resources

Sample Preparation

Inorganics

  • Milestone Ethos EZ Microwave Digestion System
  • Environmental Express Hotblocks

Organics

  • Solid Phase Extraction (SPE) of organic compounds in water samples on C18 or mixed-mode resin cartridges.
  • CEM EDGE automated solvent extraction system
  • Liquid-Solid extractions via traditional Soxhlet

Other Microbiology

  • Bio-Rad iQ5 Real Time PCR Detection System
  • Thermo Scientific Nanodrop 1000/3300
  • CO2 incubators, laminar flow hood, biological safety hood
  • -70° C Revco freezer
  • Microscopes with phase-contrast and epifluorescence capability

Other Resources

  • Two Coy anerobic glove bags
  • Two constant temperature rooms (400 sq. ft. each) maintained between 10° and 30 ± 2° C